|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat CD16a ORF mammalian expression plasmid, C-GFPSpark tag||RG80017-ACG|
|Rat CD16a ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80017-ACR|
|Rat CD16a ORF mammalian expression plasmid, C-Flag tag||RG80017-CF|
|Rat CD16a ORF mammalian expression plasmid, C-His tag||RG80017-CH|
|Rat CD16a ORF mammalian expression plasmid, C-Myc tag||RG80017-CM|
|Rat CD16a ORF mammalian expression plasmid, C-HA tag||RG80017-CY|
|Rat CD16a Gene cDNA clone plasmid||RG80017-M|
|Rat CD16a ORF mammalian expression plasmid, N-Flag tag||RG80017-NF|
|Rat CD16a ORF mammalian expression plasmid, N-His tag||RG80017-NH|
|Rat CD16a ORF mammalian expression plasmid, N-Myc tag||RG80017-NM|
|Rat CD16a ORF mammalian expression plasmid, N-HA tag||RG80017-NY|
|Rat CD16a natural ORF mammalian expression plasmid||RG80017-UT|
|Learn more about expression Vectors|
The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.
CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.