|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat LEFTY2 ORF mammalian expression plasmid, C-GFPSpark tag||RG80129-ACG|
|Rat LEFTY2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80129-ACR|
|Rat LEFTY2 ORF mammalian expression plasmid, C-Flag tag||RG80129-CF|
|Rat LEFTY2 ORF mammalian expression plasmid, C-His tag||RG80129-CH|
|Rat LEFTY2 ORF mammalian expression plasmid, C-Myc tag||RG80129-CM|
|Rat LEFTY2 ORF mammalian expression plasmid, C-HA tag||RG80129-CY|
|Rat LEFTY2 Gene cDNA clone plasmid||RG80129-G|
|Rat LEFTY2 ORF mammalian expression plasmid, N-Flag tag||RG80129-NF|
|Rat LEFTY2 ORF mammalian expression plasmid, N-His tag||RG80129-NH|
|Rat LEFTY2 ORF mammalian expression plasmid, N-Myc tag||RG80129-NM|
|Rat LEFTY2 ORF mammalian expression plasmid, N-HA tag||RG80129-NY|
|Rat LEFTY2 natural ORF mammalian expression plasmid||RG80129-UT|
|Learn more about expression Vectors|
left-right determination factor 2 (Lefty2), also known as Left-right determination factor A (Lefty A), endometrial bleeding associated factor (left-right determination, factor A transforming growth factor beta superfamily), and Transforming growth factor beta-4, is a member of the TGF-beta family of proteins. This protein is secreted and plays a role in left-right asymmetry determination of organ systems during development. Lefty2/Lefty A may also play a role in endometrial bleeding. Mutations in Lefty2/Lefty A have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. Alternative processing of this protein can yield three different products. Depletion of both Lefty1 and Lefty2 causes unchecked Nodal signaling, expansion of mesendoderm, and loss of ectoderm. The expansion of mesendoderm correlates with an extended period of rapid cellular internalization and a failure of deep-cell epiboly. The gastrulation defects of embryos depleted of Lefty1 and Lefty2 result from the deregulation of Squint signaling.