|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat INSR ORF mammalian expression plasmid, C-GFPSpark tag||RG80099-ACG|
|Rat INSR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80099-ACR|
|Rat INSR ORF mammalian expression plasmid, C-Flag tag||RG80099-CF|
|Rat INSR ORF mammalian expression plasmid, C-His tag||RG80099-CH|
|Rat INSR ORF mammalian expression plasmid, C-Myc tag||RG80099-CM|
|Rat INSR ORF mammalian expression plasmid, C-HA tag||RG80099-CY|
|Rat INSR Gene cDNA clone plasmid||RG80099-G|
|Rat INSR ORF mammalian expression plasmid, N-Flag tag||RG80099-NF|
|Rat INSR ORF mammalian expression plasmid, N-His tag||RG80099-NH|
|Rat INSR ORF mammalian expression plasmid, N-Myc tag||RG80099-NM|
|Rat INSR ORF mammalian expression plasmid, N-HA tag||RG80099-NY|
|Rat INSR natural ORF mammalian expression plasmid||RG80099-UT|
|Learn more about expression Vectors|
INSR (Insulin receptor), also known as CD220, is a transmembrane receptor that is activated by insulin. INSR belongs to theprotein kinase superfamily, and exists as a tetramer consisting of two alpha subunits and two beta subunits linked by disulfide bonds. The alpha and beta subunits are encoded by a single INSR gene, and the beta subunits pass through the cellular membrane. As the receptor for insulin with tyrosine-protein kinase activity, INSR associates with downstream mediators upon binding to insulin, including IRS1 (insulin receptor substrate 1) and phosphatidylinositol 3'-kinase (PI3K). IRS-1 binding and phosphorylation eventually leads to an increase in the high affinity glucose transporter (Glut4) molecules on the outer membrane of insulin-responsive tissues. INSR isoform long and isoform short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin, and is found as a hybrid receptor with IGF1R which also binds IGF1 in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta. Defects in Insulin Receptor/INSR are the cause of Rabson-Mendenhall syndrome (Mendenhall syndrome), insulin resistance (Ins resistance), leprechaunism (Donohue syndrome), and familial hyperinsulinemic hypoglycemia 5 (HHF5). It may also be associated with noninsulin-dependent diabetes mellitus (NIDDM).