|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Rat PRLR ORF mammalian expression plasmid, C-GFPSpark tag||RG80062-ACG|
|Rat PRLR ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80062-ACR|
|Rat PRLR ORF mammalian expression plasmid, C-Flag tag||RG80062-CF|
|Rat PRLR ORF mammalian expression plasmid, C-His tag||RG80062-CH|
|Rat PRLR ORF mammalian expression plasmid, C-Myc tag||RG80062-CM|
|Rat PRLR ORF mammalian expression plasmid, C-HA tag||RG80062-CY|
|Rat PRLR Gene cDNA clone plasmid||RG80062-M|
|Rat PRLR ORF mammalian expression plasmid, N-Flag tag||RG80062-NF|
|Rat PRLR ORF mammalian expression plasmid, N-His tag||RG80062-NH|
|Rat PRLR ORF mammalian expression plasmid, N-Myc tag||RG80062-NM|
|Rat PRLR ORF mammalian expression plasmid, N-HA tag||RG80062-NY|
|Rat PRLR natural ORF mammalian expression plasmid||RG80062-UT|
|Learn more about expression Vectors|
Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type â… cytokine receptor superfamily, and contains two fibronectin type-â…¢ domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules invloved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.