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Human GLT25D2 Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
GLT25D2Protein Product Information
Synonym:RP11-498P10.2, C1orf17, FLJ37771, FLJ37873, KIAA0584
Protein Construction:A DNA sequence encoding the human GLT25D2 (Q8IYK4) (Met 1-Ser 622) was fused with a polyhistidine tag at the C-terminus.
Species:Human
Expressed Host:Baculovirus-Insect Cells
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
GLT25D2Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met 1
Molecule Mass:The recombinant human GLT25D2 consists of 632 amino acids and predicts a molecular mass of 73.8 kDa. It migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
GLT25D2Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
12542-H08B
Background

Glycosyl transferase 25 domain 2 (GLT25D2) is a glucosyltransferase enzyme expressed only at low levels in the nervous system. Glycosyltransferases are enzymes that act as a catalyst for the transfer of a monosaccharide unit from an activated nucleotide sugar (also known as the "glycosyl donor") to a glycosyl acceptor molecule, usually an alcohol. Glycosyl transferases transfer glycosyl groups onto their substrate. Localization partially defines their function. Glt25D2 enzyme showed a strong galactosyltransferase activity toward various types of collagen and toward the serum mannose-binding lectin MBL which contains a collagen domain. 

References
  • Schegg B, et al. (2009) Core glycosylation of collagen is initiated by two beta (1-O) galactosyltransferases. Mol Cell Biol. 29 (4): 943-52.
  • Sricholpech M, et al. (2011) Lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen in osteoblast culture. J Biol Chem. 286 (11): 8846-56.
  • O'Connor DH, et al. (2009) Reverse engineering the mouse brain. Nature. 461 (7266): 923-9.
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    • Human GLT25D2 Protein (His Tag) SDS-PAGE
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