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Human ALDH3A1 Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
ALDH3A1Protein Product Information
Synonym:ALDH3, ALDHIII, MGC10406
Protein Construction:A DNA sequence encoding the human ALDH3A1 (AAH04370.1) (Met 1-His 453) was fused with a polyhistidine tag at the N-terminus.
Species:Human
Expressed Host:Baculovirus-Insect Cells
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
ALDH3A1Protein QC Testing
Purity:> 90 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human ALDH3A1 consists of 472 amino acids and has a calculated molecular mass of 52.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
ALDH3A1Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
12523-H07B
References
  • Pappa A, et al. (2003) Human aldehyde dehydrogenase 3A1 (ALDH3A1): biochemical characterization and immunohistochemical localization in the cornea. Biochem J. 376(3): 615-23.
  • Estey T, et al. (2007) ALDH3A1: a corneal crystallin with diverse functions. Experimental Eye Research. 84(1):3-12.
  • Pappa A, et al. (2003) Aldh3a1 protects human corneal epithelial cells from ultraviolet- and 4-hydroxy-2-nonenal-induced oxidative damage. Free Radical Biology and Medicine. 34(9):1178-89.
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    • Human ALDH3A1 Protein (His Tag) SDS-PAGE
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