|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse RHOA ORF mammalian expression plasmid, C-GFPSpark tag||MG52590-ACG|
|Mouse RHOA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52590-ACR|
|Mouse RHOA ORF mammalian expression plasmid, N-GFPSpark tag||MG52590-ANG|
|Mouse RHOA ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52590-ANR|
|Mouse RHOA ORF mammalian expression plasmid, C-Flag tag||MG52590-CF|
|Mouse RHOA ORF mammalian expression plasmid, C-His tag||MG52590-CH|
|Mouse RHOA ORF mammalian expression plasmid, C-Myc tag||MG52590-CM|
|Mouse RHOA ORF mammalian expression plasmid, C-HA tag||MG52590-CY|
|Mouse RHOA ORF mammalian expression plasmid, N-Flag tag||MG52590-NF|
|Mouse RHOA ORF mammalian expression plasmid, N-His tag||MG52590-NH|
|Mouse RHOA ORF mammalian expression plasmid, N-Myc tag||MG52590-NM|
|Mouse RHOA ORF mammalian expression plasmid, N-HA tag||MG52590-NY|
|Mouse RHOA Gene cDNA clone plasmid||MG52590-U|
|Mouse RHOA natural ORF mammalian expression plasmid||MG52590-UT|
|Learn more about expression Vectors|
Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.