|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse ILKAP ORF mammalian expression plasmid, C-GFPSpark tag||MG52651-ACG|
|Mouse ILKAP ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52651-ACR|
|Mouse ILKAP ORF mammalian expression plasmid, N-GFPSpark tag||MG52651-ANG|
|Mouse ILKAP ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG52651-ANR|
|Mouse ILKAP ORF mammalian expression plasmid, C-Flag tag||MG52651-CF|
|Mouse ILKAP ORF mammalian expression plasmid, C-His tag||MG52651-CH|
|Mouse ILKAP ORF mammalian expression plasmid, C-Myc tag||MG52651-CM|
|Mouse ILKAP ORF mammalian expression plasmid, C-HA tag||MG52651-CY|
|Mouse ILKAP Gene cDNA clone plasmid||MG52651-G|
|Mouse ILKAP ORF mammalian expression plasmid, N-Flag tag||MG52651-NF|
|Mouse ILKAP ORF mammalian expression plasmid, N-His tag||MG52651-NH|
|Mouse ILKAP ORF mammalian expression plasmid, N-Myc tag||MG52651-NM|
|Mouse ILKAP ORF mammalian expression plasmid, N-HA tag||MG52651-NY|
|Mouse ILKAP natural ORF mammalian expression plasmid||MG52651-UT|
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Integrin-linked kinase-associated serine/threonine phosphatase 2C, also known as ILKAP, is cytoplasm protein which belongs to the PP2C family. ILKAP contains one PP2C-like domain. ILKAP is widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues. ILKAP may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. ILKAP selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. ILKAP inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. Integrin-linked kinase ( ILK ) plays key roles in a variety of cell functions, including cell proliferation, adhesion and migration. Within the cell, ILK localizes to multiple sites, including the cytoplasm, focal adhesion complexes that mediate cell adhesion to extracellular substrates, as well as cell-cell junctions in epidermal keratinocytes. Nuclear ILK can be rapidly exported into the cytoplasm through a CRM1-dependent pathway, and its export is enhanced by the type 2C protein phosphatase ILKAP. Nuclear localization of ILK in epidermal keratinocytes is associated with increased DNA synthesis, which is sensitive to inhibition by ILKAP.