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Human SPEG / APEG-1 Protein (His Tag)

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Human SPEG Protein Product Information
Synonym:APEG1, BPEG, KIAA1297, MGC12676, SPEGalpha, SPEGbeta
Protein Construction:A DNA sequence encoding the human SPEG isoform 3 (Q15772-4) (Met 1-Glu 113) was fused with a polyhistidine tag at the C-terminus.
Expressed Host:E. coli
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human SPEG Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE
Bio-Activity:Kinase activity untested
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human SPEG consisting of 123 amino acids and has a calculated molecular mass of 14 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human SPEG Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human SPEG Protein SDS-PAGE
Human SPEG / APEG-1 Protein (His Tag) SDS-PAGE
Human SPEG / APEG-1 Protein (His Tag) SDS-PAGE
Other SPEG Recombinant Protein Products
SPEG/APEG-1 Background

Striated muscle preferentially expressed protein kinase, also known as aortic preferentially expressed protein 1, APEG-1, SPEG and KIAA1297, is a protein which belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. SPEG / APEG-1 contains two fibronectin type-III domains, nine Ig-like (immunoglobulin-like) domains, two protein kinase domains. Isoform 1 of SPEG is preferentially expressed in striated muscle. Non-kinase form such as isoform 3 of SPEG is predominantly expressed in the aorta. Isoform 3 of SPEG appears to be expressed only in highly differentiated ASMC in normal vessel walls and down-regulated in dedifferentiated ASMC. Isoform 3 of SPEG may have a role in regulating the growth and differentiation of arterial smooth muscle cells. Isoform 3 of SPEG is quickly down-regulated in response to vascular injury, when ASMC cells change from a quiescent to a proliferative phenotype.

Human SPEG/APEG-1 References
  • Hsieh C.-M., et al., 1996, J. Biol. Chem. 271:17354-17359.
  • Manjasetty B.A., et al., 2005, BMC Struct. Biol. 5:21-21.
  • Zhou,Y. et al., 2007, Anal Chem. 79 (15): 5826-37.
  • Greenman C., et al., 2007, Nature. 446:153-158.
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    Catalog: 12472-H08E-50
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