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LMAN2 / VIP36 Antibody, Mouse MAb

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Human LMAN2 Antibody Product Information
Immunogen:Recombinant Human LMAN2 / VIP36 protein (Catalog#12455-H08E)
Clone ID:02
Ig Type:Mouse IgG2a
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human LMAN2 / VIP36 (rh LMAN2 / VIP36; Catalog#12455-H08E; Q12907; Asp 45-Arg 322). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human LMAN2 Antibody Usage Guide
Specificity:Human LMAN2 / VIP36
No cross-reactivity in ELISA with
E.coli cell lysate
Application:WB, ELISA, IP

WB: 5-10 μg/ml

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human LMAN2. The detection limit for Human LMAN2 is approximately 0.078 ng/well.

IP: 4-6 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other LMAN2 Antibody Products
Human LMAN2 References
  • Nawa D, et al. (2007) Stable interaction of the cargo receptor VIP36 with molecular chaperone BiP. Glycobiology. 17(9): 913-21.
  • Shimada O, et al. (2003) Localization of VIP36 in the post-Golgi secretory pathway also of rat parotid acinar cells. J Histochem Cytochem. 51(8): 1057-63.
  • Hara-Kuge S, et al. (1999) Vesicular-integral membrane protein, VIP36, recognizes high-mannose type glycans containing alpha1--2 mannosyl residues in MDCK cells. Glycobiology. 9(8): 833-9.
  • Fullekrug J, et al. (1999) VIP36 localisation to the early secretory pathway. J Cell Sci. 112 ( Pt 17): 2813-21.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"