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Human C6 / complement component 6 HEK293 Cell Lysate (WB positive control)

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Human C6 Transfected / Overexpression Cell Lysate Product Information
Expressed Host:Human Cells
Product Description:Human Cell lysate that Human C6 / complement component 6 transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Sequence information:A DNA sequence encoding the human C6 (AAA59668.1) (Met 1-Ala 934) was fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:Cys 22
Molecule Mass:The secreted recombinant human C6 comprises 924 amino acids and has a predicted molecular mass of 104 kDa. The apparent molecular mass of rh C6 is approximately 110 kDa in SDS-PAGE under reducing conditions.
Human C6 Transfected / Overexpression Cell Lysate Usage Guide
Preparation Method:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
Stability:Samples are stable for up to twelve months from date of receipt.
Recommend Usage:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Storage Instruction:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
Application notes:Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
Human C6 References
  • Lengweiler S, et al. (1997) Elucidation of the disulfide-bonding pattern in the factor I modules of the sixth component (C6) of human complement. Biochim Biophys Acta. 1342 (1): 13-8.
  • Chakravarti DN, et al. (1989) Structural homology of complement protein C6 with other channel-forming proteins of complement. Proc Natl Acad Sci U S A. 86 (8): 2799-803.
  • DiScipio RG, et al. (1989) The molecular architecture of human complement component C6. J Biol Chem. 264 (27): 16197-206.
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    Catalog: 12426-H08HL-300
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