|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse DDOST ORF mammalian expression plasmid, C-GFPSpark tag||MG52108-ACG|
|Mouse DDOST ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52108-ACR|
|Mouse DDOST ORF mammalian expression plasmid, C-Flag tag||MG52108-CF|
|Mouse DDOST ORF mammalian expression plasmid, C-His tag||MG52108-CH|
|Mouse DDOST ORF mammalian expression plasmid, C-Myc tag||MG52108-CM|
|Mouse DDOST ORF mammalian expression plasmid, C-HA tag||MG52108-CY|
|Mouse DDOST Gene cDNA clone plasmid||MG52108-G|
|Mouse DDOST ORF mammalian expression plasmid, N-Flag tag||MG52108-NF|
|Mouse DDOST ORF mammalian expression plasmid, N-His tag||MG52108-NH|
|Mouse DDOST ORF mammalian expression plasmid, N-Myc tag||MG52108-NM|
|Mouse DDOST ORF mammalian expression plasmid, N-HA tag||MG52108-NY|
|Mouse DDOST natural ORF mammalian expression plasmid||MG52108-UT|
|Learn more about expression Vectors|
The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase) (DDOST), or 48-kDa subunit (OST48) is one of the catalytic subunits in this complex, exerts a typical type I membrane topology, containing a large luminal domain, a hydrophobic transmembrane domain and a short cytosolic peptide tail. DDOST/OST48 catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. The mammalian oligosaccharyltransferase (OST) is an oligomeric complex composed of three type I transmembrane proteins of the endoplasmic reticulum: ribophorin I (RI), ribophorin II (RII), and OST48. OST48 is not a glycoprotein and is not recognized by antibodies to either ribophorin. Like ribophorins I and II, OST48 was found to be an integral membrane protein, with the majority of the polypeptide located within the lumen of the endoplasmic reticulum (ER). OST48 does not show significant amino acid sequence homology to either ribophorin I or II. It had been found that only the luminal domain of RI contains ER retention information. The dilysine motif in OST48 functions as an ER localization motif because OST48 in which the two lysine residues are replaced by serine (OST48ss) is no longer retained in the ER and is found instead also at the plasma membrane.