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Human LFA-3 / CD58 Protein (Fc Tag)

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Human CD58 Protein Product Information
Protein Construction:A DNA sequence encoding the human CD58 (Q9BRW0) (Met1-Arg215) was expressed with the Fc region of human IgG1 at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human CD58 Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its binding ability in a functional ELISA. Immobilized human CD2-His (Cat:10982-H08H) at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.1 μg/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus CD2-His (Cat:90300-C08H) at 10 μg/ml (100 μl/well) can bind human CD58-Fc, The EC50 of human CD58-Fc is 0.04-0.10 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Phe 29
Molecule Mass:The recombinant human CD58/Fc is a disulfide-linked homodimer. The reduced monomer comprises 428 amino acids and has a predicted molecular mass of 48.5 kDa. The apparent molecular mass of the protein is approximately 68 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CD58 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human CD58 Protein SDS-PAGE
Human LFA-3 / CD58 Protein (Fc Tag) SDS-PAGE
Human LFA-3 / CD58 Protein (Fc Tag) SDS-PAGE
Other CD58 Recombinant Protein Products
LFA-3 / CD58 Background

CD53 is a member of the transmembrane 4 superfamily, also called the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. These proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD53 is a cell surface glycoprotein that is known to complex with integrins. Familial deficiency of CD53 gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. CD53 contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation.

Human LFA-3 / CD58 References
  • Rochelle JM. et al., 1993, Int Immunol. 5 (2): 209-16.
  • Virtaneva KI. et al., 1993, Immunogenetics. 37 (6): 461-5.
  • Horejsí V. et al., 1991, FEBS Lett. 288 (1-2): 1-4.
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    Catalog: 12409-H02H-50
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