|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse MST4 ORF mammalian expression plasmid, C-GFPSpark tag||MG51088-ACG|
|Mouse MST4 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG51088-ACR|
|Mouse MST4 ORF mammalian expression plasmid, N-GFPSpark tag||MG51088-ANG|
|Mouse MST4 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG51088-ANR|
|Mouse MST4 ORF mammalian expression plasmid, C-Flag tag||MG51088-CF|
|Mouse MST4 ORF mammalian expression plasmid, C-His tag||MG51088-CH|
|Mouse MST4 ORF mammalian expression plasmid, C-Myc tag||MG51088-CM|
|Mouse MST4 ORF mammalian expression plasmid, C-HA tag||MG51088-CY|
|Mouse MST4 Gene cDNA clone plasmid||MG51088-G|
|Mouse MST4 ORF mammalian expression plasmid, N-Flag tag||MG51088-NF|
|Mouse MST4 ORF mammalian expression plasmid, N-His tag||MG51088-NH|
|Mouse MST4 ORF mammalian expression plasmid, N-Myc tag||MG51088-NM|
|Mouse MST4 ORF mammalian expression plasmid, N-HA tag||MG51088-NY|
|Mouse MST4 natural ORF mammalian expression plasmid||MG51088-UT|
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MST4, also known as mammalian STE20-like protein kinase 4, is a novel member of the germinal center kinase subfamily of human Ste20-like kinases and is closely related to MST3. The 416 amino acid full-length MST4 contains a C-terminal regulatory domain and an N-terminal kinase domain, both of which are required for full activation of the kinase. MST4 is highly expressed in placenta, thymus, and peripheral blood leukocytes. MST4 specifically activates ERK but not JNK or p38 MAPK in transient transfected cells or in stable cell lines, and thus is biologically active in the activation of MEK/ERK pathway mediating cell growth and transformation. Further, MST4 kinase activity is stimulated significantly by epidermal growth factor receptor (EGFR) ligands, which are known to promote growth of certain cancer cells. Accordingly, MST4 have a potential role in signal transduction pathways involved in cancer progression. Three alternatively spliced isoform of MST4 have been isolated, and isoform 3 lacks an exon encoding kinase domain and may function as a dominant-negative regulator of the MST4 kinase.
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