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Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) (isolate JS2011-004) N ORF mammalian expression plasmid, C-His tag

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SFTSV SFTSV-N cDNA Clone Product Information
Gene_bank_ref_id:KC505125.1
RefSeq ORF Size:738bp
cDNA Description:Full length Clone DNA of Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) (isolate JS2011-004) N with C terminal His tag.
Gene Synonym:SFTSV-N
Species:SFTSV
Vector:pCMV3-C-His
Plasmid:pCMV3-AGP25448-N-His
Restriction Site:KpnI + XbaI (6kb + 0.79kb)
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.

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