|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Rat TNFRSF9 ORF mammalian expression plasmid, C-GFPSpark tag||RG80176-ACG|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80176-ACR|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-Flag tag||RG80176-CF|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-His tag||RG80176-CH|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-Myc tag||RG80176-CM|
|Rat TNFRSF9 ORF mammalian expression plasmid, C-HA tag||RG80176-CY|
|Rat TNFRSF9 Gene cDNA clone plasmid||RG80176-G|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-Flag tag||RG80176-NF|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-His tag||RG80176-NH|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-Myc tag||RG80176-NM|
|Rat TNFRSF9 ORF mammalian expression plasmid, N-HA tag||RG80176-NY|
|Rat TNFRSF9 natural ORF mammalian expression plasmid||RG80176-UT|
|Learn more about expression Vectors|
CD137 (also known as 4-1BB) is a surface co-stimulatory glycoprotein originally described as present on activated T lymphocytes, which belongs to the tumor necrosis factor (TNF) receptor superfamily. It is expressed mainly on activated CD4+ and CD8+ T cells, and binds to a high-affinity ligand (4-1BBL) expressed on several antigen-presenting cells such as macrophages and activated B cells. Upon ligand binding, 4-1BB is associated with the tumor necrosis factor receptor–associated factors (TRAFs), the adaptor protein which mediates downstream signaling events including the activation of NF-kappaB and cytokine production. 4-1BB signaling either by binding to 4-1BBL or by antibody ligation delivers signals for T-cell activation and growth, as well as monocyte proliferation and B-cell survival, and plays an important role in the amplification of T cell-mediated immune responses. In addition, CD137 and CD137L are expressed in different human primary tumor tissues, suggesting that they may influence the progression of tumors. Crosslinking of CD137 on activated T cells has shown promise in enhancing anti-tumor immune responses in murine models, and agonistic anti-CD137 antibodies are currently being tested in phase I clinical trials.