|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse TH ORF mammalian expression plasmid, C-GFPSpark tag||MG50997-ACG|
|Mouse TH ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50997-ACR|
|Mouse TH ORF mammalian expression plasmid, N-GFPSpark tag||MG50997-ANG|
|Mouse TH ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50997-ANR|
|Mouse TH ORF mammalian expression plasmid, C-Flag tag||MG50997-CF|
|Mouse TH ORF mammalian expression plasmid, C-His tag||MG50997-CH|
|Mouse TH ORF mammalian expression plasmid, C-Myc tag||MG50997-CM|
|Mouse TH ORF mammalian expression plasmid, C-HA tag||MG50997-CY|
|Mouse TH Gene cDNA clone plasmid||MG50997-G|
|Mouse TH ORF mammalian expression plasmid, N-Flag tag||MG50997-NF|
|Mouse TH ORF mammalian expression plasmid, N-His tag||MG50997-NH|
|Mouse TH ORF mammalian expression plasmid, N-Myc tag||MG50997-NM|
|Mouse TH ORF mammalian expression plasmid, N-HA tag||MG50997-NY|
|Mouse TH natural ORF mammalian expression plasmid||MG50997-UT|
|Learn more about expression Vectors|
Tyrosine hydroxylase (TH) is a rate-limiting enzyme in catecholamine synthesis. Tyrosine hydroxylase activity is modulated by protein-protein interactions with enzymes in the same pathway or the tetrahydrobiopterin pathway, structural proteins considered to be chaperones that mediate the neuron's oxidative state. It is phosphorylated at serine (Ser) residues Ser8, Ser19, Ser31 and Ser40 in vitro. The phosphorylation of tyrosine hydroxylase at Ser19 or Ser8 has no direct effect on tyrosine hydroxylase activity. As tyrosine hydroxylase (TH) catalyses the formation of L-DOPA, the rate-limiting step in the biosynthesis of DA, the Parkinson's disease (PD) can be considered as a TH-deficiency syndrome of the striatum. A direct pathogenetic role of TH has also been suggested, as the enzyme is a source of reactive oxygen species (ROS) in vitro and a target for radical-mediated oxidative injury. Recently, it has been demonstrated that L-DOPA is effectively oxidized by mammalian Tyrosine hydroxylase in vitro, possibly contributing to the cytotoxic effects of DOPA.