Quick Order

Text Size:AAA

Human CD47 Protein (Fc Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human CD47 Protein Product Information
Synonym:CD47, MER6
Protein Construction:A DNA sequence encoding the human CD47 (NP_942088.1) (Met1-Pro139) was expressed with the Fc region of human IgG1 at the C-terminus.
Species:Human
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human CD47 Protein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Bio-Activity:1. Measured by its binding ability in a functional ELISA. Immobilized human SIRPA-His (Cat:11612-H08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 10.1-23.5 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized mouse SIRPA-His (Cat:50956-M08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.05-0.13 μg/ml.
3. Measured by its binding ability in a functional ELISA. Immobilized human SIRPG-His (Cat:11828-H08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.58-1.34 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Gln 19
Molecule Mass:The recombinant human CD47/Fc is a disulfide-linked homodimer. The reduced monomer comprises 362 amino acids and has a predicted molecular mass of 40.7 kDa. The apparent molecular mass of the protein is approximately 48-60 kDa in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human CD47 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other CD47 Recombinant Protein Products
12283-H02H
CD47 Background

CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.

Human CD47 References
  • Brown EJ, et al. (2001) Integrin-associated protein (CD47) and its ligands. Trends Cell Biol. 11(3): 130-5.
  • Oldenborg PA. (2004) Role of CD47 in erythroid cells and in autoimmunity. Leuk Lymphoma. 45(7): 1319-27.
  • Kaczorowski DJ, et al. (2007) Targeting CD47: NO limit on therapeutic potential. Circ Res. 100(5): 602-3.
  • Product nameProduct name
    Size / Price
    Catalog: 12283-H02H-50
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn StockShipping instructions
    Images
    • Human CD47 Protein (Fc Tag) SDS-PAGE
    Recently Viewed Items
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"