|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse STIM1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50955-ACG|
|Mouse STIM1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50955-ACR|
|Mouse STIM1 ORF mammalian expression plasmid, C-Flag tag||MG50955-CF|
|Mouse STIM1 ORF mammalian expression plasmid, C-His tag||MG50955-CH|
|Mouse STIM1 ORF mammalian expression plasmid, C-Myc tag||MG50955-CM|
|Mouse STIM1 ORF mammalian expression plasmid, C-HA tag||MG50955-CY|
|Mouse STIM1 Gene cDNA clone plasmid||MG50955-G|
|Mouse STIM1 ORF mammalian expression plasmid, N-Flag tag||MG50955-NF|
|Mouse STIM1 ORF mammalian expression plasmid, N-His tag||MG50955-NH|
|Mouse STIM1 ORF mammalian expression plasmid, N-Myc tag||MG50955-NM|
|Mouse STIM1 ORF mammalian expression plasmid, N-HA tag||MG50955-NY|
|Mouse STIM1 natural ORF mammalian expression plasmid||MG50955-UT|
|Learn more about expression Vectors|
Stromal interaction molecule 1, also known as STIM1 and GOK, is a cell membrane, a single-pass type I membrane protein and a endoplasmic reticulum membrane protein. STIM1 / GOK is ubiquitously expressed in various human primary cells and tumor cell lines. It contains one EF-hand domain and one SAM (sterile alpha motif) domain. STIM1 / GOK plays a role in mediating Ca2+ influx following depletion of intracellular Ca2+ stores. It acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, STIM1 / GOK translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A / ORAI1. Transfection of STIM1 / GOK into cells derived from a rhabdoid tumor and from a rhabdomyosarcoma that do not express detectable levels of STIM1 can induce cell death, suggesting a possible role in the control of rhabdomyosarcomas and rhabdoid tumors. Defects in STIM1 are the cause of immune dysfunction with T-cell inactivation due to calcium entry defect type 2 (IDTICED2) which is an immune disorder characterized by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell production of cytokines, lymphadenopathy, and normal lymphocytes counts and serum immunoglobulin levels.