|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse TIGIT ORF mammalian expression plasmid, C-GFPSpark tag||MG50939-ACG|
|Mouse TIGIT ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50939-ACR|
|Mouse TIGIT ORF mammalian expression plasmid, C-Flag tag||MG50939-CF|
|Mouse TIGIT ORF mammalian expression plasmid, C-His tag||MG50939-CH|
|Mouse TIGIT ORF mammalian expression plasmid, C-Myc tag||MG50939-CM|
|Mouse TIGIT ORF mammalian expression plasmid, C-HA tag||MG50939-CY|
|Mouse TIGIT Gene cDNA clone plasmid||MG50939-G|
|Mouse TIGIT ORF mammalian expression plasmid, N-Flag tag||MG50939-NF|
|Mouse TIGIT ORF mammalian expression plasmid, N-His tag||MG50939-NH|
|Mouse TIGIT ORF mammalian expression plasmid, N-Myc tag||MG50939-NM|
|Mouse TIGIT ORF mammalian expression plasmid, N-HA tag||MG50939-NY|
|Mouse TIGIT natural ORF mammalian expression plasmid||MG50939-UT|
|Learn more about expression Vectors|
TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-10 and diminished the production of IL-12p40. In addition, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.