|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Rat YWHAE ORF mammalian expression plasmid, C-GFPSpark tag||RG80090-ACG|
|Rat YWHAE ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80090-ACR|
|Rat YWHAE ORF mammalian expression plasmid, N-GFPSpark tag||RG80090-ANG|
|Rat YWHAE ORF mammalian expression plasmid, N-OFPSpark / RFP tag||RG80090-ANR|
|Rat YWHAE ORF mammalian expression plasmid, C-Flag tag||RG80090-CF|
|Rat YWHAE ORF mammalian expression plasmid, C-His tag||RG80090-CH|
|Rat YWHAE ORF mammalian expression plasmid, C-Myc tag||RG80090-CM|
|Rat YWHAE ORF mammalian expression plasmid, C-HA tag||RG80090-CY|
|Rat YWHAE Gene cDNA clone plasmid||RG80090-G|
|Rat YWHAE ORF mammalian expression plasmid, N-Flag tag||RG80090-NF|
|Rat YWHAE ORF mammalian expression plasmid, N-His tag||RG80090-NH|
|Rat YWHAE ORF mammalian expression plasmid, N-Myc tag||RG80090-NM|
|Rat YWHAE ORF mammalian expression plasmid, N-HA tag||RG80090-NY|
|Rat YWHAE natural ORF mammalian expression plasmid||RG80090-UT|
|Learn more about expression Vectors|
YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.