|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse PLAT ORF mammalian expression plasmid, C-GFPSpark tag||MG50910-ACG|
|Mouse PLAT ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50910-ACR|
|Mouse PLAT ORF mammalian expression plasmid, C-Flag tag||MG50910-CF|
|Mouse PLAT ORF mammalian expression plasmid, C-His tag||MG50910-CH|
|Mouse PLAT ORF mammalian expression plasmid, C-Myc tag||MG50910-CM|
|Mouse PLAT ORF mammalian expression plasmid, C-HA tag||MG50910-CY|
|Mouse PLAT Gene cDNA clone plasmid||MG50910-G|
|Mouse PLAT ORF mammalian expression plasmid, N-Flag tag||MG50910-NF|
|Mouse PLAT ORF mammalian expression plasmid, N-His tag||MG50910-NH|
|Mouse PLAT ORF mammalian expression plasmid, N-Myc tag||MG50910-NM|
|Mouse PLAT ORF mammalian expression plasmid, N-HA tag||MG50910-NY|
|Mouse PLAT natural ORF mammalian expression plasmid||MG50910-UT|
|Learn more about expression Vectors|
Tissue plasminogen activator (abbreviated tPA or PLAT), is traditionally viewed as a simple serine protease whose main function is to convert plasminogen into biologically active plasmin. As a protease, tPA plays a crucial role in regulating blood fibrinolysis, in maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. tPA is synthesized and secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Proteolytic cleavage at the C-terminal side of Arg275 generates the enzyme composed of two subunits, designated as α and β chains which are held together by a single disulfide bond. Unlike the other members of the chymotrypsin family, tPA has one particular distinction in that the catalytic efficiency of the single-chain enzyme is only slightly lower than that of the proteolytically cleaved form and is therefore not a true zymogen. tPA is found not only in the blood, where its primary function is as a thrombolytic enzyme, but also in the central nervous system (CNS). It participats in a number of physiological and pathological events in the CNS, as well as the role of neuroserpin as the natural regulator of tPA's activity in these processes. Increased or decreased activity of tPA leads to hyperfibrinolysis or hypofibrinolysis, respectively. In addition, as a cytokine, tPA plays a pivotal role in the pathogenesis of renal interstitial fibrosis through diverse mechanisms. Thus, as a fibrogenic cytokine, it promotes the progression of kidney diseases.