|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse GFRA3 ORF mammalian expression plasmid, C-GFPSpark tag||MG50901-ACG|
|Mouse GFRA3 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50901-ACR|
|Mouse GFRA3 ORF mammalian expression plasmid, C-Flag tag||MG50901-CF|
|Mouse GFRA3 ORF mammalian expression plasmid, C-His tag||MG50901-CH|
|Mouse GFRA3 ORF mammalian expression plasmid, C-Myc tag||MG50901-CM|
|Mouse GFRA3 ORF mammalian expression plasmid, C-HA tag||MG50901-CY|
|Mouse GFRA3 Gene cDNA clone plasmid||MG50901-G|
|Mouse GFRA3 ORF mammalian expression plasmid, N-Flag tag||MG50901-NF|
|Mouse GFRA3 ORF mammalian expression plasmid, N-His tag||MG50901-NH|
|Mouse GFRA3 ORF mammalian expression plasmid, N-Myc tag||MG50901-NM|
|Mouse GFRA3 ORF mammalian expression plasmid, N-HA tag||MG50901-NY|
|Mouse GFRA3 natural ORF mammalian expression plasmid||MG50901-UT|
|Learn more about expression Vectors|
Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 3 (GFRA3) or GDNFRa3 is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA3 / GDNFRa3 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. The neurotrophic growth factor artemin binds selectively to GDNF family receptor α3 (GFRA3 / GDNFRa3), forming a molecular complex with the co-receptor RET which mediates downstream signaling. This signaling pathway has been demonstrated to play an important role in the survival and maintenance of nociceptive sensory neurons and in the development of sympathetic neurons.