|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse YWHAB ORF mammalian expression plasmid, C-GFPSpark tag||MG50861-ACG|
|Mouse YWHAB ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50861-ACR|
|Mouse YWHAB ORF mammalian expression plasmid, N-GFPSpark tag||MG50861-ANG|
|Mouse YWHAB ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50861-ANR|
|Mouse YWHAB ORF mammalian expression plasmid, C-Flag tag||MG50861-CF|
|Mouse YWHAB ORF mammalian expression plasmid, C-His tag||MG50861-CH|
|Mouse YWHAB ORF mammalian expression plasmid, C-Myc tag||MG50861-CM|
|Mouse YWHAB ORF mammalian expression plasmid, C-HA tag||MG50861-CY|
|Mouse YWHAB Gene cDNA clone plasmid||MG50861-G|
|Mouse YWHAB ORF mammalian expression plasmid, N-Flag tag||MG50861-NF|
|Mouse YWHAB ORF mammalian expression plasmid, N-His tag||MG50861-NH|
|Mouse YWHAB ORF mammalian expression plasmid, N-Myc tag||MG50861-NM|
|Mouse YWHAB ORF mammalian expression plasmid, N-HA tag||MG50861-NY|
|Mouse YWHAB natural ORF mammalian expression plasmid||MG50861-UT|
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14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.