|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Rat CD16a ORF mammalian expression plasmid, C-GFPSpark tag||RG80017-ACG|
|Rat CD16a ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80017-ACR|
|Rat CD16a ORF mammalian expression plasmid, C-Flag tag||RG80017-CF|
|Rat CD16a ORF mammalian expression plasmid, C-His tag||RG80017-CH|
|Rat CD16a ORF mammalian expression plasmid, C-Myc tag||RG80017-CM|
|Rat CD16a ORF mammalian expression plasmid, C-HA tag||RG80017-CY|
|Rat CD16a Gene cDNA clone plasmid||RG80017-M|
|Rat CD16a ORF mammalian expression plasmid, N-Flag tag||RG80017-NF|
|Rat CD16a ORF mammalian expression plasmid, N-His tag||RG80017-NH|
|Rat CD16a ORF mammalian expression plasmid, N-Myc tag||RG80017-NM|
|Rat CD16a ORF mammalian expression plasmid, N-HA tag||RG80017-NY|
|Rat CD16a natural ORF mammalian expression plasmid||RG80017-UT|
|Learn more about expression Vectors|
The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.
CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.