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Rat GpT2 ORF mammalian expression plasmid, N-His tag

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Rat GPT2 cDNA Clone Product Information
Gene_bank_ref_id:NM_001012057.1
RefSeq ORF Size:1569bp
cDNA Description:Full length Clone DNA of Rattus norvegicus glutamic pyruvate transaminase (alanine aminotransferase) 2 with N terminal His tag.
Gene Synonym:ALT2, Cc2-5, Gpt2
Species:Rat
Vector:pCMV3-N-His
Plasmid:
Restriction Site:
Tag Sequence:His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Sequence Description:
Sequencing primers:T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
Promoter:Enhanced CMV mammalian cell promoter
Application:Stable or Transient mammalian expression
Antibiotic in E.coli:Kanamycin
Antibiotic in mammalian cell:Hygromycin
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
His Tag Info

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.

Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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Background

Alanine aminotransferase (ALT), also known as glutamate pyruvate transaminase (Gpt), is a pyridoxal enzyme which catalyses the reversible interconversion of L-alanine and 2-oxoglutalate to pyruvate and L-glutamate, and plays a key role in the intermediary metabolism of glucose and amino acids. As a key enzyme for gluconeogenesis, Gpt is a widely-used serum marker for liver injury. Two ALT isoenzymes have been identified, ALT1 and ALT2 (GPT1 and GPT2), which are encoded by separate genes and share significant sequence homology, but differ in their expression patterns. Gpt1 is widely distributed and mainly expressed in intestine, liver, fat tissues, colon, muscle, and heart, in the order of high to low expression level, whereas Gpt2 expression is more restricted, mainly in liver, muscle, brain, and white adipose tissue. It has been reported that hepatic ALT2 protein is approximately four times higher in male rats than in female rats.

References
  • Matthews, C.C. et al., 2002, Genomics. 79: 445-450.
  • Jadhao, S.B. et al., 2004, Hepatology. 39: 1297-1302.
  • Schindhelm, R.K. et al., 2005, Eur. J.Clin. Invest. 35:369-374.
  • Lindblom, P. et al., 2007, Arch Biochem Biophys. 466 (1): 66-77.
  • Liu, L.et al., 2008, Protein Expr Purif. 60 (2): 225-231.
  • Yang, R. Z. et al., 2009, Hepatology. 49 (2): 598-607.
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    Catalog: RG80002-NH
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