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Human FES Kinase / Feline sarcoma oncogene Protein (His & GST Tag)

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Human FES Protein Product Information
Synonym:FPS, FES
Protein Construction:A DNA sequence encoding the human FES (P07332-1) (Met 1-Arg 822) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human FES Protein QC Testing
Purity:> 75 % as determined by SDS-PAGE
Bio-Activity:The specific activity was determined to be 200 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human FES/GST chimera consists of 1060 amino acids and has a calculated molecular mass of 121 kDa. It migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human FES Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other FES Recombinant Protein Products
FES/Feline sarcoma oncogene Background

Proto-oncogene tyrosine-protein kinase Fes/Fps, also known as Proto-oncogene c-Fes, Proto-oncogene c-Fps, Feline sarcoma oncogene, FES and FPS, is a protein which contains one FCH domain, one protein kinase domain and one SH2 domain. FES is a non-receptor protein tyrosine kinase expressed in hematopoietic progenitors and differentiated myeloid cells. FES is observed in the nuclear, granular and plasma membrane fractions of primary human neutrophils and the myeloid leukemia cell line, HL-60. The nuclear localization is confirmed by immunocytochemistry of neutrophils. FES has been implicated in granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-3 (IL-3) and erythropoietin signal transduction. FES has tyrosine-specific protein kinase activity and that activity is required for maintenance of cellular transformation. FES is also involved in normal hematopoiesis. Its chromosomal location has linked it to a specific translocation event identified in patients with acute promyelocytic leukemia.

Human FES/Feline sarcoma oncogene References
  • Bowden DW, et al.,1991, Nucleic acids Res 19 (15): 4311.
  • Yates,K.E. et al., 1995, Oncogene. 10 (6):1239-42.
  • Jücker, M, et al.,1997, J. Biol. Chem.  272 (4): 2104-9.
  • Smithgall,T.E. et al., 1998, Crit Rev Oncog. 9 (1):43-62.
  • Lionberger, et al.,2000, Cancer Res. 60 (4): 1097-103.
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    Catalog: 12214-H20B-50
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