|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse PRKCI ORF mammalian expression plasmid, C-GFPSpark tag||MG50788-ACG|
|Mouse PRKCI ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50788-ACR|
|Mouse PRKCI ORF mammalian expression plasmid, N-GFPSpark tag||MG50788-ANG|
|Mouse PRKCI ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50788-ANR|
|Mouse PRKCI ORF mammalian expression plasmid, C-Flag tag||MG50788-CF|
|Mouse PRKCI ORF mammalian expression plasmid, C-His tag||MG50788-CH|
|Mouse PRKCI ORF mammalian expression plasmid, C-Myc tag||MG50788-CM|
|Mouse PRKCI ORF mammalian expression plasmid, C-HA tag||MG50788-CY|
|Mouse PRKCI Gene cDNA clone plasmid||MG50788-G|
|Mouse PRKCI ORF mammalian expression plasmid, N-Flag tag||MG50788-NF|
|Mouse PRKCI ORF mammalian expression plasmid, N-His tag||MG50788-NH|
|Mouse PRKCI ORF mammalian expression plasmid, N-Myc tag||MG50788-NM|
|Mouse PRKCI ORF mammalian expression plasmid, N-HA tag||MG50788-NY|
|Mouse PRKCI natural ORF mammalian expression plasmid||MG50788-UT|
|Learn more about expression Vectors|
Protein kinase C iota type, also known as Atypical protein kinase C-lambda/iota, aPKC-lambda/iota and PRKCI, is a cytoplasm, membrane and nucleus protein which belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCI contains one AGC-kinase C-terminal domain, one OPR domain, one phorbol-ester/DAG-type zinc finger and one protein kinase domain. PRKCI is predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. It is highly expressed in non-small cell lung cancers. PRKCI is a calcium-independent, phospholipid-dependent, serine- and threonine-specific kinase. It may play a role in the secretory response to nutrients. PRKCI is involved in cell polarization processes and the formation of epithelial tight junctions. It is implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. PRKCI functions in both pro- and anti-apoptotic pathways. It functions in the RAC1/ERK signaling required for transformed growth. PRKCI plays a role in microtubule dynamics through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). PRKCI might be a target for novel lipid activators that are elevated during nutrient-stimulated insulin secretion.