|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse PTK6 ORF mammalian expression plasmid, C-Flag tag||MG50710-CF|
|Mouse PTK6 ORF mammalian expression plasmid, C-His tag||MG50710-CH|
|Mouse PTK6 ORF mammalian expression plasmid, C-Myc tag||MG50710-CM|
|Mouse PTK6 ORF mammalian expression plasmid, C-HA tag||MG50710-CY|
|Mouse PTK6 ORF mammalian expression plasmid, N-Flag tag||MG50710-NF|
|Mouse PTK6 ORF mammalian expression plasmid, N-His tag||MG50710-NH|
|Mouse PTK6 ORF mammalian expression plasmid, N-Myc tag||MG50710-NM|
|Mouse PTK6 ORF mammalian expression plasmid, N-HA tag||MG50710-NY|
|Mouse PTK6 natural ORF mammalian expression plasmid||MG50710-UT|
|Learn more about expression Vectors|
Tyrosine kinase (PTKs) is a protein that carry out tyrosine phosphorylation, which play a fundamental role in cell proliferation, survival, adhesion, and motility and have also been demenstrated to mediate malignant cell transformation. Overexpression of this protein in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Two classes of PTKs are present in cells: the transmembrane receptor PTKs and the non-receptor PTKs. Tyrosine kinase(PTKs)-6/ BRK is a cytoplasmic non-receptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Tyrosine kinase(PTKs)-6/ BRK has been shown to undergo autophosphorylation. It has been found that the constitutive expression of the tyrosine kinase(PTKs)-6/ BRK is in a large proportion of cutaneous T-cell lymphomas and other transformed T- and B-cell populations. State BRK expression was also induced in normal T-cells. In clinical, the cytoplasmic tyrosine kinase PTK6 (BRK) shows elevated expression in approximately two-thirds of primary breast tumours, and is implicated in EGF receptor-dependent signalling and epithelial tumorigenesis.