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Human MARK3 / CTAK1 / EMK-2 Protein (His & GST Tag)

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Human MARK3 Protein Product Information
Synonym:CTAK1, KP78, PAR1A
Protein Construction:A DNA sequence encoding full length of human MARK3 (AAH24773.1) (Met 1-Leu 729) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human MARK3 Protein QC Testing
Purity:> 88 % as determined by SDS-PAGE
Bio-Activity:The specific activity was determined to be 7 nmol/min/mg using synthetic CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human MARK3/GST chimera consists of 967 amino acids and has a calculated molecular mass of 109.3 kDa. It migrates as an approximately 120 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, 0.25mM DTT, 0.1mM PMSF, 0.1mM EDTA, pH 7.4, 10% gly
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human MARK3 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human MARK3 Protein SDS-PAGE
Human MARK3 / CTAK1 / EMK-2 Protein (His & GST Tag) SDS-PAGE
Human MARK3 / CTAK1 / EMK-2 Protein (His & GST Tag) SDS-PAGE
Other MARK3 Recombinant Protein Products
MARK3/C-TAK1 Background

MAP / microtubule affinity-regulating kinase 3, also known as C-TAK1, cTAK1, Cdc25C-associated protein kinase 1, ELKL motif kinase 2, Protein kinase STK10, Ser/Thr protein kinase PAR-1, Serine/threonine-protein kinase p78, MARK3, CTAK1 and EMK2, is a ubiquitous expressed protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and MARK subfamily. MARK3 contains one KA1 (kinase-associated) domain, one protein kinase domain and one UBA domain. The Par-1 / MARK protein kinases play a pivotal role in establishing cellular polarity. This family of kinases contains a unique domain architecture, in which a ubiquitin-associated (UBA) domain is located C-terminal to the kinase domain. MARKs / PAR-1 are common regulators of cell polarity that are conserved from nematode to human. All of these kinases have a highly conserved C-terminal domain, which is termed the kinase-associated domain 1 (KA1).

Human MARK3/C-TAK1 References
  • Kato,T. et al., 2001, Neoplasia.3 (1):4-9.
  • Tochio,N. et al., 2006, Protein Sci. 15 (11):2534-43.
  • Murphy,J.M. et al., 2007, Proc Natl Acad Sci USA.104 (36):14336-41.
  • de Leng,W.W. et al., 2007,Clin Genet. 72 (6):568-73.
  • Chia,C.Y. et al., 2010, IUBMB Life. 62 (3):200-3.
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    Catalog: 12181-H20B-20
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