|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse GSK3B ORF mammalian expression plasmid, C-GFPSpark tag||MG50650-ACG|
|Mouse GSK3B ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50650-ACR|
|Mouse GSK3B ORF mammalian expression plasmid, N-GFPSpark tag||MG50650-ANG|
|Mouse GSK3B ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50650-ANR|
|Mouse GSK3B ORF mammalian expression plasmid, C-Flag tag||MG50650-CF|
|Mouse GSK3B ORF mammalian expression plasmid, C-His tag||MG50650-CH|
|Mouse GSK3B ORF mammalian expression plasmid, C-Myc tag||MG50650-CM|
|Mouse GSK3B ORF mammalian expression plasmid, C-HA tag||MG50650-CY|
|Mouse GSK3B Gene cDNA clone plasmid||MG50650-M|
|Mouse GSK3B ORF mammalian expression plasmid, N-Flag tag||MG50650-NF|
|Mouse GSK3B ORF mammalian expression plasmid, N-His tag||MG50650-NH|
|Mouse GSK3B ORF mammalian expression plasmid, N-Myc tag||MG50650-NM|
|Mouse GSK3B ORF mammalian expression plasmid, N-HA tag||MG50650-NY|
|Mouse GSK3B natural ORF mammalian expression plasmid||MG50650-UT|
|Learn more about expression Vectors|
GSK3B is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It Contains 1 protein kinase domain, and is expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in GSK3B gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. GSK3B participates in the Wnt signaling pathway. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. GSK3B also plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. GSK3B phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. It may be required for early embryo development and neuron differentiation.