|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse EPHA2 ORF mammalian expression plasmid, C-GFPSpark tag||MG50586-ACG|
|Mouse EPHA2 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50586-ACR|
|Mouse EPHA2 ORF mammalian expression plasmid, C-Flag tag||MG50586-CF|
|Mouse EPHA2 ORF mammalian expression plasmid, C-His tag||MG50586-CH|
|Mouse EPHA2 ORF mammalian expression plasmid, C-Myc tag||MG50586-CM|
|Mouse EPHA2 ORF mammalian expression plasmid, C-HA tag||MG50586-CY|
|Mouse EPHA2 Gene cDNA clone plasmid||MG50586-G|
|Mouse EPHA2 ORF mammalian expression plasmid, N-Flag tag||MG50586-NF|
|Mouse EPHA2 ORF mammalian expression plasmid, N-His tag||MG50586-NH|
|Mouse EPHA2 ORF mammalian expression plasmid, N-Myc tag||MG50586-NM|
|Mouse EPHA2 ORF mammalian expression plasmid, N-HA tag||MG50586-NY|
|Mouse EPHA2 natural ORF mammalian expression plasmid||MG50586-UT|
|Learn more about expression Vectors|
Eph receptor A2 (Ephrin type-A receptor 2 or EphA2) is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. The Eph receptors' corresponding family of ligands are the ephrins anchored to cell surfaces. The ephrins and Eph receptors are implicated as positional labels that may guide the development of neural topographic maps. They have also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization. The large family of ligands and receptors may make a major contribution to the accurate spatial patterning of connections and cell position in the nervous system. Furthermore, elevated expression of Eph receptors and ephrin ligands is associated with tumors and associated tumor vasculature, suggesting the Eph receptors and ephrin ligands also play critical roles in tumor angiogenesis and tumor growth. Unlike most Eph kinases, which are primarily expressed during development, EphA2 is primarily found in adult human epithelial cells. The cellular functions of EphA2 may be regulating cell growth, survival, migration, and angiogenesis.Unlike other receptor tyrosine kinases, ligand binding is not necessary for EphA2. Rather, the ligand appears to regulate EphA2 subcellular localization and its interactions with downstream adapter and signaling proteins. Eph receptor A2(EphA2) has been demonstrated to critically regulate tumor cell growth, migration and invasiveness. Eph receptor A2(EphA2) is frequently overexpressed and functionally altered in aggressive tumor cells, and that these changes promote metastatic character.