After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

RELA / Transcription factor p65 / NFkB p65 Antibody, Mouse MAb

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
RELAAntibody Product Information
Immunogen:Recombinant Human RELA / Transcription factor p65 protein (Catalog#12054-H09E)
Clone ID:04
Ig Type:Mouse IgG2a
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RELA / Transcription factor p65 (rh RELA / Transcription factor p65; Catalog#12054-H09E; Q04206-1; Met 1-Tyr 306). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
RELAAntibody Usage Guide
Specificity:Human RELA / Transcription factor p65
Application:WB, ICC/IF, FCM, IF

WB: 5-10 μg/mL

ICC/IF: 15-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
RELA / Transcription factor p65 / NFkB p65 Antibody, Mouse MAb, Flow cytometric
[Click to enlarge image]
Flow cytometric analysis of Purified anti-Human RelA/NFkB p65 on Jurkat cells (Left panel) and HeLa cells (Right panel).
RELA / Transcription factor p65 / NFkB p65 Antibody, Mouse MAb, Immunofluorescence
[Click to enlarge image]
Immunofluorescence staining of human RELA (NF-KB p65) in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-human RELA monoclonal antibody (10 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

RELA (v-rel reticuloendotheliosis viral oncogene homolog A), also known as Nuclear factor NF-kappa-B p65 subunit, or Transcription factor p65, is a transcription factor expressed in growth plate chondrocytes where it facilitates chondrogenesis. The v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA) gene encodes the major component of the NF-?B complex. NF-kappaB is a generic name for an evolutionarily conserved transcription-factor system that contributes to the mounting of an effective immune response but is also involved in the regulation of cell proliferation, development, and apoptosis. The implication of NF-kappaB in central biological processes and its extraordinary connectivity to other signaling pathways raise a need for highly controlled regulation of NF-kappaB activity at several levels. The mammalian Rel/NF-kappaB family of transcription factors, including RelA, c-Rel, RelB, NF-kappaB1 (p50 and its precursor p105), and NF-kappaB2 (p52 and its precursor p100), plays a central role in the immune system by regulating several processes ranging from the development and survival of lymphocytes and lymphoid organs to the control of immune responses and malignant transformation.

  • Hashimoto R, et al. (2011) Variants of the RELA gene are associated with schizophrenia and their startle responses. Neuropsychopharmacology. 36(9): 1921-31.
  • Vallabhapurapu S, et al. (2009) Regulation and function of NF-kappaB transcription factors in the immune system. Annu Rev Immunol. 27: 693-733.
  • Schmitz ML, et al. (2004) NF-kappaB: a multifaceted transcription factor regulated at several levels. Chembiochem. 5(10): 1348-58.
  • Related Products
    Product nameProduct name