|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokarfyotic expression systems.
|Rat CLPS ORF mammalian expression plasmid, C-GFPSpark tag||RG80729-ACG|
|Rat CLPS ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80729-ACR|
|Rat CLPS ORF mammalian expression plasmid, C-Flag tag||RG80729-CF|
|Rat CLPS ORF mammalian expression plasmid, C-His tag||RG80729-CH|
|Rat CLPS ORF mammalian expression plasmid, C-Myc tag||RG80729-CM|
|Rat CLPS ORF mammalian expression plasmid, C-HA tag||RG80729-CY|
|Rat CLPS ORF mammalian expression plasmid, N-Flag tag||RG80729-NF|
|Rat CLPS ORF mammalian expression plasmid, N-His tag||RG80729-NH|
|Rat CLPS ORF mammalian expression plasmid, N-Myc tag||RG80729-NM|
|Rat CLPS ORF mammalian expression plasmid, N-HA tag||RG80729-NY|
|Rat CLPS Gene cDNA clone plasmid||RG80729-U|
|Rat CLPS natural ORF mammalian expression plasmid||RG80729-UT|
|Learn more about expression Vectors|
Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.