|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse TWSG1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50162-ACG|
|Mouse TWSG1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50162-ACR|
|Mouse TWSG1 ORF mammalian expression plasmid, C-Flag tag||MG50162-CF|
|Mouse TWSG1 ORF mammalian expression plasmid, C-His tag||MG50162-CH|
|Mouse TWSG1 ORF mammalian expression plasmid, C-Myc tag||MG50162-CM|
|Mouse TWSG1 ORF mammalian expression plasmid, C-HA tag||MG50162-CY|
|Mouse TWSG1 Gene cDNA clone plasmid||MG50162-M|
|Mouse TWSG1 ORF mammalian expression plasmid, N-Flag tag||MG50162-NF|
|Mouse TWSG1 ORF mammalian expression plasmid, N-His tag||MG50162-NH|
|Mouse TWSG1 ORF mammalian expression plasmid, N-Myc tag||MG50162-NM|
|Mouse TWSG1 ORF mammalian expression plasmid, N-HA tag||MG50162-NY|
|Mouse TWSG1 natural ORF mammalian expression plasmid||MG50162-UT|
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TWSG1 belongs to the twisted gastrulation protein family. TWSG1 from different species are functionally equivalent. In contrast to Drosophila where TWSG1 expression is limited to early embryos, expression of TWSG1 is found throughout mouse and human development. Mutations in the TWSG1 gene cause at least some of the cells on the dorsal half of the embryo to adopt more ventral cell fates. This is thought to involve gradients of the signaling molecule decapentaplegic. TWSG1 may function as a bone morphogenetic protein signalling agonist or antagonize these activities. It can dislodge latent bone morphogenetic proteins and thus provides a permissive signal that allows high BMP signaling in the embryo. TWSG1 is a cofactor in the antagonism of chordin to BMP signaling. It also binds both the vertebrate Decapentaplegic ortholog BMP4 and chordin and forms ternary complexes. Meanwhile, TWSG1 increases binding of chordin to BMP4, potentiates the ability of chordin to induce secondary axes in Xenopus embryos, and enhances chordin cleavage by vertebrate proteases related to tolloid at a site poorly used in the absence of TWSG1. The presence of TWSG1 enhances the secondary axis-inducing activity of 2 products of chordin cleavage.