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B2M / beta-2 microglobulin Antibody, Mouse MAb

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Human B2M Antibody Product Information
Immunogen:Recombinant Human B2M / beta-2 microglobulin protein (Catalog#11976-H08H)
Clone ID:2E6H3
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human B2M / beta-2 microglobulin (rh B2M / beta-2 microglobulin; Catalog#11976-H08H; NP_004039.1; Met 1-Met 119). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human B2M Antibody Usage Guide
Specificity:Human B2M / beta-2 microglobulin
No cross-reactivity in ELISA with
Human cell lysate (293 cell line)
Application:ELISA, IHC-P

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human B2M. The detection limit for Human B2M is approximately 0.0195 ng/well.

IHC-P: 10-30 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human B2M Antibody IHC Application Image
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Caption:
Immunochemical staining of human B2M in human esophageal carcinoma with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections).
B2M / beta-2 microglobulin Antibody, Mouse MAb, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human B2M in human ovarian cancer (from 2 donors) with mouse monoclonal antibody (15 µg/mL, formalin-fixed paraffin embedded sections).
B2M / beta-2 microglobulin Antibody, Mouse MAb, Immunohistochemistry
Other B2M Antibody Products
B2M/beta-2 microglobulin Background

B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M(β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M(β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M(β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to theα3 chain. Unlikeα3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M(β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M(β2 microglobulin) can indicate non-progression of HIV.

Human B2M/beta-2 microglobulin References
  • Poulik MD, et al. (1979) Beta 2-Microglobulin: methods and clinical applications. CRC Ctit Rev Clin Lab Sci. 10(3): 225-45.
  • Poulik MD, et al. (1975) Beta2-Microglobulins. Contemp Top Mol Immunol. 4: 157-204.
  • Berggard I. (1976) Beta2-Microglobulins: isolation, properties, and distribution. Fed Proc. 35(5): 1167-70.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"