|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse SERPIND1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50121-ACG|
|Mouse SERPIND1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50121-ACR|
|Mouse SERPIND1 ORF mammalian expression plasmid, C-Flag tag||MG50121-CF|
|Mouse SERPIND1 ORF mammalian expression plasmid, C-His tag||MG50121-CH|
|Mouse SERPIND1 ORF mammalian expression plasmid, C-Myc tag||MG50121-CM|
|Mouse SERPIND1 ORF mammalian expression plasmid, C-HA tag||MG50121-CY|
|Mouse SERPIND1 Gene cDNA clone plasmid||MG50121-M|
|Mouse SERPIND1 ORF mammalian expression plasmid, N-Flag tag||MG50121-NF|
|Mouse SERPIND1 ORF mammalian expression plasmid, N-His tag||MG50121-NH|
|Mouse SERPIND1 ORF mammalian expression plasmid, N-Myc tag||MG50121-NM|
|Mouse SERPIND1 ORF mammalian expression plasmid, N-HA tag||MG50121-NY|
|Mouse SERPIND1 natural ORF mammalian expression plasmid||MG50121-UT|
|Learn more about expression Vectors|
SerpinD1, also known as heparin cofactor II (HCâ…¡), is a member of Serpin superfamily of the serine proteinase inhibitors. HCII is a glycoprotein in human plasma that inhibits thrombin and chymotrypsin, and the rate of inhibition of thrombin is rapidly increased by Dermatan sulfate (DS), heparin (H) and glycosaminoglycans(GAG). The stimulatory effect of glycosaminoglycans on the inhibition is mediated, in part, by the N-terminal acidic domain of HCII. Interestingly, a C-terminal His-tagged recombinant HCII exhibits enhanced activity of thrombin inhibition. It has been suggested that HCII plays an unique and important role in vascular homeostasis, and accordingly mutations in this gene or congenital HCII deficiency is potentially associated with thrombosis. HCII specifically inhibits thrombin action at the site of vascular wall injury and HCII-thrombin complexes have been detected in human plasma. HCII protects against thrombin-induced vascular remodeling in both humans and mice and suggest that HCII is a predictive biomarker and therapeutic target for atherosclerosis. SerpinD1 also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner.