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Human DAPK1 / DAP Kinase 1 Protein (aa 1-363, His & GST Tag)

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Human DAPK1 Protein Product Information
Synonym:RP11-104M22.1, MGC126323, MGC126324
Protein Construction:A DNA sequence encoding the N-terminal segment of human DAPK1 (P53355-1) (Met 1-Leu 363) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human DAPK1 Protein QC Testing
Purity:> 85 % as determined by SDS-PAGE
Bio-Activity:The specific activity was determined to be 20 nmol/min/mg using synthetic R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK) as substrate.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human DAPK1 (aa 1-363)/GST chimera consists of 600 amino acids and has a calculated molecular mass of 69.4 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human DAPK1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human DAPK1 Protein SDS-PAGE
Human DAPK1 / DAP Kinase 1 Protein (aa 1-363, His & GST Tag) SDS-PAGE
Human DAPK1 / DAP Kinase 1 Protein (aa 1-363, His & GST Tag) SDS-PAGE
Other DAPK1 Recombinant Protein Products
DAPK1 Background

Death-associated protein kinase 1, also known as DAP kinase 1, DAPK1 and DAPK, is a cytoplasm protein which belongs to the protein kinase superfamily, CAMK Ser / Thr protein kinase family and DAP kinase subfamily. DAPK1 contains ten ANK repeats, one death domain and one protein kinase domain. DAPK1 is a calcium / calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. DAPK1 gene is a candidate tumor suppressor (TSG) and the abnormal methylation of DAPK1 gene has been found in many carcinomas. DAPK1 over-expression can induce cell apoptosis and inhibit tumor cell metastasis. DAPK1 gene over-expression could suppress PGCl3 cells malignant phenotype, inhibit PGCl3 cells growth, invasive, migration and adhesion ability, upregulate p53 gene and downregulate bcl-2 gene. Loss of activity of death-associated protein kinase 1 ( DAPK1 ) may be an independent factor affecting survival of non-small cell lung cancer patients. DAPK1 promoter methylation might play a significant role in the progression of chronic myeloid leukemia ( CML ).

Human DAPK1 References
  • Zhang,H.T. et al., 2004, Ai Zheng. 23 (5):497-501.
  • Martoriati,A. et al., 2005, Oncogene. 24 (8):1461-6.
  • Li,Y. et al., 2006, Hum Mol Genet. 15 (17): 2560-8.
  • Martinez-Glez,V. et al., 2007, Neoplasma. 54 (2):123-6.
  • Raval,A. et al., 2007, Cell. 129 (5):879-90.
  • Gade,P. et al., 2009, Int J Cancer. 125 (7):1566-74.
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    Catalog: 11966-H20B-50
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