|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
|Mouse DDOST ORF mammalian expression plasmid, C-GFPSpark tag||MG52108-ACG|
|Mouse DDOST ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG52108-ACR|
|Mouse DDOST ORF mammalian expression plasmid, C-Flag tag||MG52108-CF|
|Mouse DDOST ORF mammalian expression plasmid, C-His tag||MG52108-CH|
|Mouse DDOST ORF mammalian expression plasmid, C-Myc tag||MG52108-CM|
|Mouse DDOST ORF mammalian expression plasmid, C-HA tag||MG52108-CY|
|Mouse DDOST Gene cDNA clone plasmid||MG52108-G|
|Mouse DDOST ORF mammalian expression plasmid, N-Flag tag||MG52108-NF|
|Mouse DDOST ORF mammalian expression plasmid, N-His tag||MG52108-NH|
|Mouse DDOST ORF mammalian expression plasmid, N-Myc tag||MG52108-NM|
|Mouse DDOST ORF mammalian expression plasmid, N-HA tag||MG52108-NY|
|Mouse DDOST natural ORF mammalian expression plasmid||MG52108-UT|
|Learn more about expression Vectors|
The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase) (DDOST), or 48-kDa subunit (OST48) is one of the catalytic subunits in this complex, exerts a typical type I membrane topology, containing a large luminal domain, a hydrophobic transmembrane domain and a short cytosolic peptide tail. DDOST/OST48 catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. The mammalian oligosaccharyltransferase (OST) is an oligomeric complex composed of three type I transmembrane proteins of the endoplasmic reticulum: ribophorin I (RI), ribophorin II (RII), and OST48. OST48 is not a glycoprotein and is not recognized by antibodies to either ribophorin. Like ribophorins I and II, OST48 was found to be an integral membrane protein, with the majority of the polypeptide located within the lumen of the endoplasmic reticulum (ER). OST48 does not show significant amino acid sequence homology to either ribophorin I or II. It had been found that only the luminal domain of RI contains ER retention information. The dilysine motif in OST48 functions as an ER localization motif because OST48 in which the two lysine residues are replaced by serine (OST48ss) is no longer retained in the ER and is found instead also at the plasma membrane.