|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse ARSA ORF mammalian expression plasmid, C-GFPSpark tag||MG50018-ACG|
|Mouse ARSA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50018-ACR|
|Mouse ARSA ORF mammalian expression plasmid, C-Flag tag||MG50018-CF|
|Mouse ARSA ORF mammalian expression plasmid, C-His tag||MG50018-CH|
|Mouse ARSA ORF mammalian expression plasmid, C-Myc tag||MG50018-CM|
|Mouse ARSA ORF mammalian expression plasmid, C-HA tag||MG50018-CY|
|Mouse ARSA Gene cDNA clone plasmid||MG50018-M|
|Mouse ARSA ORF mammalian expression plasmid, N-Flag tag||MG50018-NF|
|Mouse ARSA ORF mammalian expression plasmid, N-His tag||MG50018-NH|
|Mouse ARSA ORF mammalian expression plasmid, N-Myc tag||MG50018-NM|
|Mouse ARSA ORF mammalian expression plasmid, N-HA tag||MG50018-NY|
|Mouse ARSA natural ORF mammalian expression plasmid||MG50018-UT|
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Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH-dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in diagnosis of lung and central nervous system cancer.