pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony screening (i.e., a-complementation), as well as a pUC origin of replication.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Rat RHOA ORF mammalian expression plasmid, C-GFPSpark tag||RG80547-ACG|
|Rat RHOA ORF mammalian expression plasmid, C-OFPSpark / RFP tag||RG80547-ACR|
|Rat RHOA ORF mammalian expression plasmid, C-Flag tag||RG80547-CF|
|Rat RHOA ORF mammalian expression plasmid, C-His tag||RG80547-CH|
|Rat RHOA ORF mammalian expression plasmid, C-Myc tag||RG80547-CM|
|Rat RHOA ORF mammalian expression plasmid, C-HA tag||RG80547-CY|
|Rat RHOA ORF mammalian expression plasmid, N-Flag tag||RG80547-NF|
|Rat RHOA ORF mammalian expression plasmid, N-His tag||RG80547-NH|
|Rat RHOA ORF mammalian expression plasmid, N-Myc tag||RG80547-NM|
|Rat RHOA ORF mammalian expression plasmid, N-HA tag||RG80547-NY|
|Rat RHOA Gene cDNA clone plasmid||RG80547-U|
|Rat RHOA natural ORF mammalian expression plasmid||RG80547-UT|
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Transforming protein RhoA, also known as Rho cDNA clone 12, Ras homolog gene family member A, RHOA and ARH12, is a cell membrane and cytoplasm protein which belongs to the small GTPase superfamily and Rho family. The Rho family of small GTPases plays a key role in the dynamic regulation of the actin cytoskeleton that underlies various important cellular functions such as shape changes, migration, and polarity. RHOA / ARH12 is part of a larger family of related proteins known as the Ras superfamily; proteins involved in the regulation and timing of cell division. RHOA / ARH12 is a small GTPase protein known to regulate the actin cytoskeleton in the formation of stress fibers. It acts upon two known effector proteins: ROCK1 (Rho-associated, coiled-coil containing protein kinase 1) and DIAPH1 ( diaphanous homolog 1 (Drosophila) ). RHOA / ARH12 regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. RHOA / ARH12 serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. RHOA / ARH12 may be an activator of PLCE1. It is activated by ARHGEF2, which promotes the exchange of GDP for GTP.