|Datasheet||Specific References||Reviews||Related Products||Protocols|
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
The actual HA tag is as follows: 5' TAC CCA TAC GAT GTT CCA GAT TAC GCT 3' or 5' TAT CCA TAT GAT GTT CCA GAT TAT GCT 3' The amino acid sequence is: YPYDVPDYA.
|Mouse BCL2L1 ORF mammalian expression plasmid, C-GFPSpark tag||MG50012-ACG|
|Mouse BCL2L1 ORF mammalian expression plasmid, C-OFPSpark / RFP tag||MG50012-ACR|
|Mouse BCL2L1 ORF mammalian expression plasmid, N-GFPSpark tag||MG50012-ANG|
|Mouse BCL2L1 ORF mammalian expression plasmid, N-OFPSpark / RFP tag||MG50012-ANR|
|Mouse BCL2L1 ORF mammalian expression plasmid, C-Flag tag||MG50012-CF|
|Mouse BCL2L1 ORF mammalian expression plasmid, C-His tag||MG50012-CH|
|Mouse BCL2L1 ORF mammalian expression plasmid, C-Myc tag||MG50012-CM|
|Mouse BCL2L1 ORF mammalian expression plasmid, C-HA tag||MG50012-CY|
|Mouse BCL2L1 Gene cDNA clone plasmid||MG50012-M|
|Mouse BCL2L1 ORF mammalian expression plasmid, N-Flag tag||MG50012-NF|
|Mouse BCL2L1 ORF mammalian expression plasmid, N-His tag||MG50012-NH|
|Mouse BCL2L1 ORF mammalian expression plasmid, N-Myc tag||MG50012-NM|
|Mouse BCL2L1 ORF mammalian expression plasmid, N-HA tag||MG50012-NY|
|Mouse BCL2L1 natural ORF mammalian expression plasmid||MG50012-UT|
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B-cell lymphoma-extra large (Bcl-xl) is a transmembrane molecule in the mitochondria. Bcl-xL (BCL2L1) , belongs to the Bcl-2 family. Members of the bcl-2 family encode proteins that function either to promote or to inhibit apoptosis. Antiapoptotic members such as Bcl-2 and Bcl-xL prevent PCD in response to a wide variety of stimuli to take part in cancer survival. Conversely, proapoptotic proteins, exemplified by Bax and Bak, can accelerate death and in some instances are sufficient to cause apoptosis independent of additional signals. The crystal and solution structures of a Bcl-2 family member, Bcl-xL is like this: The structures consist of two central, primarily hydrophobic α-helices, which are surrounded by amphipathic helices. A 60-residue loop connecting helices αl and α2 was found to be flexible and non-essential for anti-apoptotic activity. Bcl-xL is chareacterized as important factors in autophagy, inhibiting Beclin 1-mediated autophagy by binding to Beclin 1. In addition, Beclin 1, Bcl-2 and Bcl-xL can cooperate with Atg5 or Ca2+ to regulate both autophagy and apoptosis. Bcl-xL is also implicated in anoxia induced cell death. The pathway is initiated by the loss of function of the prosurvival Bcl-2 family members Mcl-1 and Bcl-2 / Bcl-XL, resulting in Bax- or Bak-dependent release of cytochrome c and subsequent caspase-9-dependent cell death. Thus, Bcl-xL, the well-characterized apoptosis guards, appears to be important in cell death.