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Human ERN1 / IRE1 Protein (aa 465-977)

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Human ERN1 Protein Product Information
Synonym:ERN1
Protein Construction:A DNA sequence encoding the human ERN1 (O75460-1) (Pro 465-Leu 977) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
Species:Human
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human ERN1 Protein QC Testing
Purity:> 80 % as determined by SDS-PAGE
Bio-Activity:1. Kinase activity untested
2. Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Gly
Molecule Mass:The secreted recombinant human ERN1 consists of 515 amino acids and predicts a molecular mass of 58.3 KDa. The apparent molecular mass of the protein is approximately 65 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human ERN1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human ERN1 Protein SDS-PAGE
Human ERN1 / IRE1 Protein (aa 465-977) SDS-PAGE
Other ERN1 Recombinant Protein Products
11905-HNCB
Human ERN1/IRE1 References
  • Trentmann,S.M. et al., 2000, Plant Mol Biol. 44 (1):11-25.
  • Iwawaki T., et al., 2001, Nat. Cell Biol. 3:158-64.
  • Liu C.Y., et al., 2003, J. Biol. Chem. 278:17680-7.
  • Oppermann F.S., et al., 2009, Mol. Cell. Proteomics 8:1751-64.
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    Catalog: 11905-HNCB-20
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"