After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!

Quick Order

Text Size:AAA

Human ERN1 / IRE1 Protein (aa 465-977, His & GST Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human ERN1 Protein Product Information
Synonym:FLJ30999, IRE1, IRE1P, IRE1a, MGC163277, MGC163279, hIRE1p
Protein Construction:A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Species:Human
Expressed Host:Baculovirus-Insect Cells
Shipping:Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.
Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Human ERN1 Protein QC Testing
Purity:> 80 % as determined by SDS-PAGE
Bio-Activity:1. Kinase activity untested
2. Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human ERN1/GST chimera consists of 750 amino acids and has a calculated molecular mass of 86 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
Formulation:Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human ERN1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other ERN1 Recombinant Protein Products
11905-H20B
Human ERN1/IRE1 References
  • Trentmann,S.M. et al., 2000, Plant Mol Biol. 44 (1):11-25.
  • Iwawaki T., et al., 2001, Nat. Cell Biol. 3:158-64.
  • Liu C.Y., et al., 2003, J. Biol. Chem. 278:17680-7.
  • Oppermann F.S., et al., 2009, Mol. Cell. Proteomics 8:1751-64.
  • Product nameProduct name
    Size / Price
    Catalog: 11905-H20B-20
    List Price:   (Save )
    Price:      [How to order]
    AvailabilityIn StockShipping instructions
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"