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Human IFNL3 / IL28B / Interleukin-28B Protein (His Tag)

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Human IL28B Protein Product Information
Synonym:IFNL3, IL-28B, IL28C
Protein Construction:A DNA sequence encoding the human IL28B (NP_742151.2) (Met 1-Val 196) was expressed, with a polyhistidine tag at the C-terminus.
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human IL28B Protein QC Testing
Purity:> 96 % as determined by SDS-PAGE
Bio-Activity:1. Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus (VSV) . The EC50 for this effect is typically 2-6 ng/mL .
2. Measured by its binding ability in a functional ELISA . Immobilized human IL28B at 20 μg/ml (100 μl/well) can bind human IL10RB with a linear ranger of 3.125-25 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Val 22
Molecule Mass:The recombinant human IL28B consists of 186 amino acids and predictes a molecular mass of 21 kDa. In SDS-PAGE under reducing conditions, rhIL28B migrates as an approximately 23 kDa band.
Formulation:Lyophilized from sterile PBS, 500mM NaCl, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human IL28B Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other IL28B Recombinant Protein Products
IL-28B/IFN-lambda-3 Background

Interleukin-28B (IL-28B) also known as Interferon lambda-3 and IFN-lambda-3, belongs to the type III interferon family of cytokines and are highly similar to IL-29. IL-28B belongs to the newly described interferon lambda (IFNλ) family of cytokines. IL-28B is a cytokine with immunomodulatory activity. It functions in Up-regulating MHC class I antigen expression. IL-28B displays potent antiviral activity and antitumor activity. This cytokine serves as ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-28B, like IL-12, is capable of robustly enhancing adaptive immunity. Moreover, we describe for the first time how IL-28B reduces regulatory T-cell populations during DNA vaccination, whereas IL-12 increases this cellular subset. We also show that IL-28B, unlike IL-12, is able to increase the percentage of splenic CD8+ T cells in vaccinated animals, and that these cells are more granular and have higher antigen-specific cytolytic degranulation compared with cells taken from animals that received IL-12 as an adjuvant.

Human IL-28B/IFN-lambda-3 References
  • Ge D, et al.. (2009) Genetic variation in IL28B predicts hepatitis C treatment-induced viral clearance. Nature. 461(7262): 399-401.
  • Morrow MP, et al.. (2009) Comparative ability of IL-12 and IL-28B to regulate Treg populations and enhance adaptive cellular immunity. Blood. 113(23): 5868-77.
  • Sheppard P, et al.. (2003) IL-28, IL-29 and their class II cytokine receptor IL-28R. Nat Immunol. 4(1): 63-8.
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    Catalog: 11890-H08H-20
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