After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

Human Neurexin-3-beta / NRXN3 HEK293 Cell Lysate (WB positive control)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
Human NRXN3 Transfected / Overexpression Cell Lysate Product Information
Expressed Host:Human Cells
Product Description:Human Cell lysate that Human NRXN3 / Neurexin-3-beta transfected / overexpressed for Western blot (WB) positive control. The whole cell lysate is provided in 1X Sample Buffer (1X modified RIPA buffer+1X SDS loading buffer).
Sequence information:A DNA sequence encoding the human NRXN3 isoform 2 (NP_620426.2) extracellular domain (Met 1-Thr 357) was fused with the Fc region of human IgG1 at the C-terminus.
Predicted N Terminal:Ser 36
Molecule Mass:The secreted recombinant human NRXN3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 563 amino acids and predictes a molecular mass of 61.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh NRXN3/Fc monomer is approximately 75-85 kDa due to glycosylation.
Human NRXN3 Transfected / Overexpression Cell Lysate Usage Guide
Preparation Method:Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis Buffer:Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Quality Control Testing:12.5% SDS-PAGE Stained with Coomassie Blue after protein purification.
Stability:Samples are stable for up to twelve months from date of receipt.
Recommend Usage:1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
Storage Buffer:1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
Storage Instruction:Store at 4℃. After re-dissolution, aliquot and store at -80℃.
Application notes:Western blot (WB): Use at an assay dependent dilution.
Other Applications: Not tested.
Optimal dilutions/concentrations should be determined by the end user.
NRXN3/Neurexin 3 Background

Neurexin-3-beta, also known as Neurexin III-beta and NRXN3, is a single-pass type I  membrane protein which belongs to the neurexin family. It contains one laminin G-like domain. It is a neuronal cell surface protein that may be involved in cell recognition and cell adhesion. Neurexins are a family of proteins that function in the vertebrate nervous system as cell adhesion molecules and receptors. They are encoded by several unlinked genes of which two, NRXN1 and NRXN3, are among the largest known human genes. Three of the genes ( NRXN1, NRXN2, NRXN3 ) utilize two alternate promoters and include numerous alternatively spliced exons to generate thousands of distinct mRNA transcripts and protein isoforms. The majority of transcripts are produced from the upstream promoter and encode alpha-neurexin isoforms; a much smaller number of transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. The alpha-neurexins contain EGF-like sequences and laminin G domains, and have been shown to interact with neurexophilins. The beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. NRXN3 have been linked to genetic predisposition towards a number of conditions such as alcohol or drug addiction, or obesity.

Human NRXN3/Neurexin 3 References
  • Occhi G. et al., 2002, Biochem. Biophys. Res. Commun. 298:151-5.
  • Rowen L, et al., 2002, Genomics. 79 (4): 587-97.
  • Olsen J.V. et al., 2006, Cell 127:635-48.
  • Hishimoto A, et al., 2007, Human Molecular Genetics.16 (23): 2880-91.
  • Oppermann F.S. et al., 2009, Mol. Cell. Proteomics 8:1751-64. 
  • Size / Price
    Catalog: 11843-H02HL-300
    List Price:   (Save )
    Price:      [How to order]
    Availability2 weeksShipping instructions