After search, choose a molecule or a kind of categories listed in the left to narrow down your filter. If you have any problems, please contact us!
Text Size:AAA

C4.4A / LYPD3 Antibody, Rabbit MAb

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
LYPD3Antibody Product Information
Immunogen:Recombinant Human C4.4A / LYPD3 protein (Catalog#11836-H08H)
Clone ID:213
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Human C4.4A / LYPD3 (rh C4.4A / LYPD3; Catalog#11836-H08H; NP_055215.2; Met 1-His 286).
LYPD3Antibody Usage Guide
Specificity:Human C4.4A / LYPD3
Application:WB, ELISA, ICC/IF, IF

WB: 5-10 μg/mL

This antibody can be used at 5-10 μg/mL with the appropriate secondary reagents to detect Human LYPD3 in WB.

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human LYPD3. The detection limit for Human LYPD3 is approximately 0.0049 ng/well.

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
C4.4A / LYPD3 Antibody, Rabbit MAb, Immunofluorescence
[Click to enlarge image]
Immunofluorescence staining of Human LYPD3 in PC3 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with rabbit anti-Human LYPD3 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cell membrane.

Ly6 / PLAUR domain-containing protein 3, also known as GPI-anchored metastasis-associated protein C4.4A homolog, Matrigel-induced gene C4 protein, MIG-C4 and LYPD3, is a cell membrane protein which contains two UPAR/Ly6 domains. Human LYPD3 contains two UPAR/Ly6 domains. LYPD3 is expressed in placenta, skin and urothelium. It is found in suprabasal keratinocytes of chronic wounds. Weak expression of LYPD3 is found in esophagus and peripheral blood mononuclear cells. It is found in the majority of primary and metastatic transitional cell carcinomas (TCCs) and as well in breast cancer tissues, but not in adjacent normal tissues. High expression of LYPD3 is found in the tumor component of some noninvasive superficial lesions and in invasive and metastatic urothelial cancers. LYPD3 is up-regulated in migrating keratinocytes during epithelisation of incisional skin wounds. LYPD3 supports cell migration. It may be involved in urothelial cell-matrix interactions. It may also be involved in tumor progression

  • Smith B.A., et al., 2001, Cancer Res. 61:1678-85.
  • Wuerfel J., et al., 2001, Gene 262:35-41.
  • Clark H.F., et al., 2003, Genome Res. 13:2265-70.
  • Fletcher G.C., et al., 2003, Br. J. Cancer 88:579-85.
  • Hansen L.V., et al., 2004, Biochem. Eng. J. 380:845-57.
  • Related Products
    Product nameProduct name
    Size / Price
    List Price: $170.00  (Save $0.00)
    Price:$170.00      [How to order]
    AvailabilityIn Stock
    • C4.4A / LYPD3 Antibody, Rabbit MAb, Immunofluorescence
    • C4.4A / LYPD3 Antibody, Rabbit MAb, Western blot
    Recently Viewed Items