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Human TWF1 / PTK9 / Twinfilin-1 Protein (His & GST Tag)

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Human TWF1 Protein Product Information
Synonym:A6, MGC23788, MGC41876, PTK9
Protein Construction:A DNA sequence encoding the human TWF1 isoform 4 (Q12792-4) (Met 1-Asp 252) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:E. coli
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human TWF1 Protein QC Testing
Purity:> 84 % as determined by SDS-PAGE
Endotoxin:Please contact us for more information.
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human TWF1/GST chimera consists of 494 amino acids and has a calculated molecular mass of 57 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 50mM Tris, 500mM NaCl, pH 8.0
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human TWF1 Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Human TWF1 Protein SDS-PAGE
Human TWF1 / PTK9 / Twinfilin-1 Protein (His & GST Tag) SDS-PAGE
Other TWF1 Recombinant Protein Products
TWF1/PTK9 Background

Twinfilin-1, also known as Protein A6, Protein tyrosine kinase 9, TWF1 and PTK9, is a cytoplasm protein which belongs to the actin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1 / PTK9 ) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1, a second protein with approximately 65% sequence identity to twinfilin-1 exists in mouse and humans. TWF1 / PTK9 is expressed at high levels in the colon, testis, ovary, prostate and lung. It is expressed at lower levels in the brain, bladder and heart. It is not detected in liver. TWF1 / PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. TWF1 / PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.

Human TWF1/PTK9 References
  • Beeler JF, et al.,1994, Mol Cell Biol 14 (2): 982-8.
  • Palmgren S, et al., 2002, J. Cell. Sci. 115 (Pt 5): 881-6.
  • Vartiainen MK, et al.,2003, J. Biol. Chem. 278 (36): 34347-55.
  • Hassel S, et al.,2004, Proteomics 4 (5): 1346-58.
  • Moseley,J.B. et al., 2006, J Cell Sci. 119 (Pt 8):1547-57.
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    Catalog: 11829-H20E-100
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