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Human Semaphorin 4D / SEMA4D / CD100 Protein (His Tag)

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Human SEMA4D Protein Product Information
Synonym:XXyac-YM68G7.1, C9orf164, CD100, FLJ33485, FLJ34282, FLJ39737, FLJ46484, M-sema-G, MGC169138, MGC169141, SEMAJ, coll-4
Protein Construction:A DNA sequence encoding the human SEMA4D isoform 1 (Q92854-1) extracellular domain (Met 1-Arg 734) was expressed, with a polyhistidine tag at the C-terminus.
Species:Human
Expressed Host:Human Cells
Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Human SEMA4D Protein QC Testing
Purity:> 92 % as determined by SDS-PAGE
Bio-Activity:Measured by its binding ability in a functional ELISA.
Immobilized human SEMA4D-His at 10 μg/ml (100 μl/well) can bind mouse PTPRC-Fc (Cat:50713-M02H1) with a linear range of 0.625-5 μg/ml.
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met 22
Molecule Mass:The recombinant human SEMA4D consists of 725 amino acids and predictes a molecular mass of 80.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSEMA4D is approximately 110 kDa due to glycosylation.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Human SEMA4D Protein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Other SEMA4D Recombinant Protein Products
11825-H08H
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Catalog: 11825-H08H-20
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"