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Human MTDH / lyric / metadherin Protein (His & GST Tag)

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MTDHProtein Product Information
Synonym:3D3, AEG-1, AEG1, LYRIC, LYRIC/3D3
Protein Construction:A DNA sequence encoding the human MTDH (AAH45642.1) (Met 1-Thr 582) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host:Baculovirus-Insect Cells
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
MTDHProtein QC Testing
Purity:> 82 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Met
Molecule Mass:The recombinant human MTDH/GST chimera consists of 819 amino acids and has a calculated molecular mass of 91.7 kDa. It migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
Formulation:Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
MTDHProtein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Metadherin (MTDH), also known as protein LYRIC or astrocyte elevated gene-1 protein (AEG-1), has emerged as an important oncogene protein that is overexpressed in all cancers analyzed so far. MTDH has been demonstrated to play a potential role in several significant aspects of tumor progression. The presence of the lung-homing domain of MTDH on tumor cells at the cell surface where it would be available to bind to vascular targets during metastasis has been confirmed. It has been reported that overexpression of MTDH is associated with progression of disease and poorer prognosis in breast cancer. It has been reported that MTDH overexpression could significantly enhance the invasion and migration of breast cancer cells by inducing epithelial-mesenchymal transition (EMT) that is a process in which polarized epithelial cells are converted into motile mesenchymal cells. Thus, during cancer development, MTDH is conducive to tumor dissemination and metastatic spread.

  • Yoo BK, et al. (2011) Astrocyte elevated gene-1 (AEG-1): A multifunctional regulator of normal and abnormal physiology. Pharmacol Ther.130 (1) : 1-8.
  • Li XY, et al. (2011) Metadherin enhances the invasiveness of breast cancer cells by inducing epithelial to mesenchymal transition. Cancer Science. 102 (6) : 1151-7.
  • Su P, et al. (2010) Immunohistochemical analysis of Metadherin in proliferative and cancerous breast tissue. Diagnostic Pathology. 5: 38.
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